In the Time Difference Of Arrival(TDOA) location system，it will produce a TDOA location ambiguity when receiving and positioning multiple radar radiation sources in a short distance. A double- time combined algorithm by using the independent position information of the moment before and after is proposed to eliminate the TDOA location ambiguity solution effectively，and then achieving high precision of multiple radar radiation sources. Analysis and simulation results show that the double-time combined algorithm in TDOA location system can solve the multiple radiation sources range ambiguity problem effectively.