To cope with the performance deterioration of communication system with narrow-band interference, an interference suppression scheme based on oversampling and Blind Sources Separation (BSS) in single channel is proposed. By exploiting the baseband structural features of narrow-band interference and communication signal, a BSS model is formed by oversampling and serial-to-parallel conversion. Then Independent Component Analysis(ICA) is utilized to solve the BSS problem and to recover the information symbols of communication signal. Simulation results further demonstrate that the scheme is effective to suppress narrow-band interference.