The preparation of magnetic shielding films by electrodeposition cannot only precisely control the chemical composition and thickness of the films, but also form films on the surface of complex geometry. In this paper, iron-nickel-copper-iron-nickel multilayer composite magnetic shielding films were prepared by electrodeposition device, and the effects of magnetic field intensity, thickness and high temperature treatment on the shielding effectiveness of magnetic shielding films were investigated. The experimental results show that the shielding effectiveness of 50 μm and 100 μm samples increases with the increase of magnetic field intensity, while that of 200 μm samples increases first and then decreases; in the 4-16 Oe magnetic field, the bigger the thickness, the better the shielding effect of the film; the shielding effectiveness of all thickness of the magnetic shielding film will be reduced under 100 ℃.